diff --git a/gui/settings.ui b/gui/settings.ui
index c3b9c25c7..924d15b54 100644
--- a/gui/settings.ui
+++ b/gui/settings.ui
@@ -292,17 +292,28 @@
          <property name="title">
           <string>Python binary (leave this empty to use python in the PATH)</string>
          </property>
-         <layout class="QHBoxLayout" name="horizontalLayout">
-          <item>
-           <widget class="QLineEdit" name="mEditPythonPath"/>
-          </item>
-          <item>
-           <widget class="QPushButton" name="mBtnBrowsePythonPath">
-            <property name="text">
-             <string>...</string>
-            </property>
-           </widget>
-          </item>
+         <layout class="QVBoxLayout" name="verticalLayout_13">
+           <item>
+             <layout class="QHBoxLayout" name="horizontalLayout">
+              <item>
+               <widget class="QLineEdit" name="mEditPythonPath"/>
+              </item>
+              <item>
+               <widget class="QPushButton" name="mBtnBrowsePythonPath">
+                <property name="text">
+                 <string>...</string>
+                </property>
+               </widget>
+              </item>
+             </layout>
+           </item>
+           <item>
+             <widget class="QLabel" name="mPythonPathWarning">
+              <property name="text">
+               <string></string>
+              </property>
+             </widget>
+           </item>
          </layout>
         </widget>
        </item>
diff --git a/gui/settingsdialog.cpp b/gui/settingsdialog.cpp
index 04c637816..1c7490d16 100644
--- a/gui/settingsdialog.cpp
+++ b/gui/settingsdialog.cpp
@@ -23,6 +23,7 @@
 #include <QListWidgetItem>
 #include <QSettings>
 #include <QFileDialog>
+#include <QFileInfo>
 #include <QThread>
 #include "settingsdialog.h"
 #include "applicationdialog.h"
@@ -41,6 +42,7 @@ SettingsDialog::SettingsDialog(ApplicationList *list,
     mTranslator(translator)
 {
     mUI.setupUi(this);
+    mUI.mPythonPathWarning->setStyleSheet("color: red");
     QSettings settings;
     mTempApplications->copy(list);
 
@@ -56,6 +58,7 @@ SettingsDialog::SettingsDialog(ApplicationList *list,
     mUI.mShowStatistics->setCheckState(boolToCheckState(settings.value(SETTINGS_SHOW_STATISTICS, false).toBool()));
     mUI.mShowErrorId->setCheckState(boolToCheckState(settings.value(SETTINGS_SHOW_ERROR_ID, false).toBool()));
     mUI.mEditPythonPath->setText(settings.value(SETTINGS_PYTHON_PATH, QString()).toString());
+    validateEditPythonPath();
     mUI.mEditMisraFile->setText(settings.value(SETTINGS_MISRA_FILE, QString()).toString());
 
 #ifdef Q_OS_WIN
@@ -69,6 +72,9 @@ SettingsDialog::SettingsDialog(ApplicationList *list,
     mCurrentStyle = new CodeEditorStyle(CodeEditorStyle::loadSettings(&settings));
     manageStyleControls();
 
+    connect(mUI.mEditPythonPath, SIGNAL(textEdited(const QString &)),
+            this, SLOT(validateEditPythonPath()));
+
     connect(mUI.mButtons, &QDialogButtonBox::accepted, this, &SettingsDialog::ok);
     connect(mUI.mButtons, &QDialogButtonBox::rejected, this, &SettingsDialog::reject);
     connect(mUI.mBtnAddApplication, SIGNAL(clicked()),
@@ -194,6 +200,28 @@ void SettingsDialog::saveCheckboxValue(QSettings *settings, QCheckBox *box,
     settings->setValue(name, checkStateToBool(box->checkState()));
 }
 
+void SettingsDialog::validateEditPythonPath()
+{
+    const auto pythonPath = mUI.mEditPythonPath->text();
+    if (pythonPath.isEmpty()) {
+        mUI.mEditPythonPath->setStyleSheet("");
+        mUI.mPythonPathWarning->hide();
+        return;
+    }
+
+    QFileInfo pythonPathInfo(pythonPath);
+    if (!pythonPathInfo.exists() ||
+        !pythonPathInfo.isFile() ||
+        !pythonPathInfo.isExecutable()) {
+        mUI.mEditPythonPath->setStyleSheet("QLineEdit {border: 1px solid red}");
+        mUI.mPythonPathWarning->setText(tr("The executable file \"%1\" is not available").arg(pythonPath));
+        mUI.mPythonPathWarning->show();
+    } else {
+        mUI.mEditPythonPath->setStyleSheet("");
+        mUI.mPythonPathWarning->hide();
+    }
+}
+
 void SettingsDialog::addApplication()
 {
     Application app;
diff --git a/gui/settingsdialog.h b/gui/settingsdialog.h
index 8c66f8b84..1fcede7f9 100644
--- a/gui/settingsdialog.h
+++ b/gui/settingsdialog.h
@@ -103,6 +103,9 @@ protected slots:
     */
     void ok();
 
+    /** @brief Slot for validating input value in @c editPythonPath */
+    void validateEditPythonPath();
+
     /**
     * @brief Slot for adding a new application to the list
     *